ZEISS Microscopy

Imaging the Near-to-Native State: Recent Advances in TEM Lamella Preparation and Volume Imaging under Cryogenic Conditions

Imaging the Near-to-Native State: Recent Advances in TEM Lamella Preparation and Volume Imaging under Cryogenic Conditions

Cryogenic microscopy is an emerging technique for the structural analysis of macromolecules in a close to native state. Recent developments in cryogenic preservation and imaging technologies now allow us to combine cryogenic close-to-native-state preservation of whole cells and tissues, and structural analysis of macromolecules in their cellular context. These vitrified samples can be imaged using a range light and electron microscopy techniques, with each revealing complementary information. However, cryogenic microscopy presents users with complex challenges, such as time-consuming preparation and imaging procedures, devitrification, ice contamination or loss of samples – and even more challenges if the user wants to correlate data across imaging modalities.

In this webinar we will demonstrate how the new ZEISS Correlative Cryo Workflow overcomes these challenges by seamlessly connecting widefield, laser scanning, and focused ion beam scanning electron microscopy. The Correlative Cryo Workflow is fully optimized for the needs of correlative cryogenic workflows, from localization of fluorescent macromolecules to high-contrast volume imaging and on-grid lamella thinning for cryo electron tomography, and this webinar will illustrate how it can simplify the workflow and enhance the characterisation of vitrified samples.

Dr. Kevin Grant

Product Application Sales Specialist
ZEISS Research Microscopy Solutions

Dr. Ruth Chalmers-Redman

Global Business Development
Life Science Electron Microscopy, Cryo, Bio XRM
ZEISS Research Microscopy Solutions
 

Key Learnings

  • The ZEISS Correlative Cryo Workflow uniquely connects widefield, laser scanning, and focused ion beam scanning electron microscopy in a seamless and easy-to-use procedure
  • The ZEISS Correlative Cryo Workflow eliminates time-consuming preparation and imaging procedures and helps overcome some critical challenges that are encountered when generating samples for single particle analysis (SPA)

February 22, 2021 | 2:00PM AEST

Monday

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