Fluorescence microscopy has been a critical tool in answering complex biological questions since its invention over 100 years ago. Modern fluorescence techniques and instrumentation continue to evolve enabling increasing advances in sensitivity, resolution, and speed allowing us to peer deeply into the dynamic processes of living cells and whole organisms. In this webinar, we will discuss the basics of fluorescence microscopy and give some useful guides into sample preparation, fluorescence labelling and state of the art fluorescence imaging techniques used by today's life science researchers
- Avoid refractive index mismatches
- Spectral overlap can be minimised by optimising filter sets and fluorophores
Shannon obtained his PhD from The University of Sydney in 2019, where he studied the oxidative stress pathways leading to cataract formation. To further his knowledge of ocular diseases and oxidative stress, he undertook postdoctoral training at The John Curtin School of Medical Research. In 2020, he joined the ZEISS ANZ team as a product application specialist in light microscopy.